Saturday, May 18, 2019
Bacterias & Prokaryotes
Archaea is considered to be another nation, besides Prokaryotes (which entangle bacteria) and eukaryotes (which include plants, animals, fungi and protests). Eukaryotes cause a well-defined nucleus, membrane cell structure. On the other hand prokaryotes have a well-defined cell wall and contain an irregular nucleus. Archaea was defined in the 1970s, and it was observed that they break throughd at high temperatures (above 100 degrees centigrade), pressure or salt concentrations and is known as extremophiles. They may produce methane fellate as part of metabolism.They were found in regions other than which other organisms cannot survive such as geysers, inunct wells, deep ocean, hypersaline waters, hot springs, etc, and hence could be helping to maintain the ecological niche. As they can survive only in in truth high temperatures in which other forms of life cannot exist, they were considred to be absent in the charitable body. However, scientists atomic number 18 considering t hat Archaea domain can in fact be present in the human body and can cause disease. Under a normal microscope viewed under standard conditions, Archaea would appear as small dots, as they are inactive at this room conidtions (B.Waggoner & B. R. Speers. 1994). With the help of an electron microscope can the Archaea domain can be examined. There are several other techniques of examining the Archaea species. Molecular techniques seem to be very effective in detecting and isolating Archaea as their genetic material can be place (especially PCR). The presence of lipids present in the Archaea cell can be determined with the help of gas chromatography and cumulation spectroscopy. Fluorescent in situ hybridization is also utilized in some instances to study the relationship amid the genetic material of Archaea and the presence of diseases.Immunological methods such as in situ immunofluorescence and immunohistochemistry are being studied as to how they could be utilized in detecting Archae a. They are of limited use as there are some issues with the antigen produced by Archaea (P. B. Eckburg. 2003 & E. F. DeLong, 1999). References B. Waggoner & B. R. Speer. Introduction to the Archaea, Lifes extremists, 1994, UCMP. 28 Sep. 2007 http//www. ucmp. berkeley. edu/archaea/archaea. hypertext markup language B. Waggoner & B. R. Speer. Archaea Morphology, 1994, UCMP. 28 Sep. 2007 http//www. ucmp. berkeley.edu/archaea/archaeamm. html Edward F. DeLong, Lance Trent Taylor, Terence L. Marsh, and Christina M. Preston. Visualization and Enumeration of Marine Planktonic Archaea and Bacteria by Using Polyribonucleotide Probes and Fluorescent In Situ Hybridization. Applied and Environmental Microbiology 65. 12 (1999). http//aem. asm. org/cgi/content/abstract/65/12/5554 Paul B. Eckburg, Paul W. Lepp, and David A. Relman. Archaea and Their capability Role in Human Disease. Infection and Immunity 71. 2 (2003). http//iai. asm. org/cgi/content/full/71/2/591
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